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A5588
Sigma
Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse
clone HIS-1, purified immunoglobulin, buffered aqueous glycerol solution
| Synonym: | Monoclonal Anti-polyHistidine |
Description
| Immunogen | recombinant polyHistidine tagged fusion protein. |
| Legal Information | This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany. |
| Specificity | The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. |
| Physical form | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
Properties
| antibody form | purified immunoglobulin |
| clone | HIS-1 (monoclonal) |
| form | buffered aqueous glycerol solution |
| application(s) | immunoblotting: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
| isotype | IgG2a |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 1 |
